Development and characterization of a vector system based on the simian adenovirus type 25

Technological versatility and the humoral cellular immune response induction capacity have conditioned wide spread of adenoviral vectors as vaccine gene therapy drugs. However, vaccination with Sputnik V made a significant portion population to types 5 26 (Ad5 Ad26) recombinant human adenovirus vectors, which are some most frequently used bases for candidate vaccines. Today, designers tend select alternative serotypes platforms develop vaccines against new pathogens on. A good example is simian type 25 (SAd25), belongs subgroup E. It genetically distant from Ad5 exhibits extremely low seroprevalence in beings, makes it an appealing vector. The purpose this work was design study platform based on 25. We relied advanced methods molecular biology virology construct make adenoviruses; phylogenetic analysis context enabled bioinformatic methods. resulting vector can effectively replicate itself HEK293 cell line (human embryonic kidney cells). This substantiates expediency further investigation into SAd25 development prevention various infectious diseases.